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Image Search Results
Journal: Frontiers in cellular and infection microbiology
Article Title: Carbohydrate-binding protein from stinging nettle as fusion inhibitor for SARS-CoV-2 variants of concern.
doi: 10.3389/fcimb.2022.989534
Figure Lengend Snippet: FIGURE 2 Antiviral activity of UDA against live SARS-CoV-2 virus in Vero E6 and U87.ACE2+ cells. Cells were exposed to clinical isolates of SARS-CoV-2, i.e., variants Wuhan-Hu-1 (A), 20A.EU2 (B), Gamma (C) or GFP-expressing Wuhan-Hu-1 (D) in the absence or presence of UDA. (A, B) SARS- CoV-2 replication was assessed by RT-qPCR analysis of the viral copy numbers of the N gene in the supernatant at day 3 post infection (p.i.). RT-qPCR data were used to calculate the % inhibition of viral replication and to plot a concentration-response curve for UDA. Graphs show data of 3 independent experiments with 2 technical replicates each (mean ± SD; n=6). (C) Cells were collected at 40h p.i. and stained intracellularly for the viral N protein. Histogram plots show mean fluorescence intensity (MFI) values of N expression in noninfected (Cell Control; grey), infected (Virus Control; blue) and UDA-treated infected (red) Vero E6 cells from a representative experiment. Single cell analysis was performed on 8,000 – 10,000 cells by flow cytometry. The numbers in each plot refer to the percentage of cells that stained positive for N (i.e., infected cells). The dashed grey histogram plot represents the background signal from the non-infected cell control. (D) Pictures, taken at 2 days post infection, show GFP expression in the infected U87.ACE2+ cells. Representative pictures from a biological replicate out of two are shown. The values between brackets refer to the percentage GFP+ area (relative to the virus control); mean ± SD (n=2).
Article Snippet: Human Embryonic Kidney 293T (HEK293T) cells (cat n° CRL-3216),
Techniques: Activity Assay, Virus, Expressing, Quantitative RT-PCR, Infection, Inhibition, Concentration Assay, Staining, Control, Single-cell Analysis, Cytometry